| Item No. | Products | Specifications |
|---|---|---|
| C-001 | Cardiosight®, small hiPSC-Derived cardiomyocytes | ≥2.5 × 10⁶ cells |
| C-002 | Cardiosight®, small hiPSC-Derived cardiomyocytes | ≥5.0 × 10⁶ cells |
| CMS-001A | Cardiosight®Advanced Basic Culture Medium (CM-010A, 30 ml) Cardiosight®Advanced Patch (50X) (CS-010A, 0.6 ml) Cardiosight®Advanced Maintenance Basic Medium (CM-001A, 100 ml) Cardiosight®Advanced Maintenance Supplement (100X) (CS-001A, 1 ml) | About 30+100 ml |
| CMS-002A | Cardiosight®Advanced Basic Culture Medium (CM-020A, 45 ml) Cardiosight®Advanced Patch (50X) (CS-020A, 0.9 ml) Cardiosight®Advanced Maintenance Basic Medium (CM-002A, 200 ml) Cardiosight®Advanced Maintenance Supplement (100X) (CS-002A, 2 ml) | About 45+200 ml |
Morphology
12 well culture dish images (magnified 100x) of Cardiosight® at 1 day, 5 days, 1 week, and 2 weeks after resuscitation. The board density of Cardiosight® is 150000 cells per square meter. Please refer to Cardiosight for detailed instructions® Product manual* MEA testing has optimized the board density, which may differ from the cell shape shown in the above figure.
Images of Cardiosight® on 48 well Axion MEA plates at 1, 3, 5, and 7 days after resuscitation. Please refer to Cardiosight® for detailed instructions Application Manual (Axion Maestro MEA).
Cardiosight® rapidly recovers after resuscitation, begins to spontaneously beat after 3 days, and stabilizes over time (45-60 bpm). It can be used for testing after 7 days.
Features
MLC2V
MLC2A
Merged
Connexin43
cTnT
Merged
Cardiosight® expresses genes specific to ventricular cardiomyocytes and possesses normal contractile function and structural features. The high expression of gap junction protein Connexin43 in Cardiosight® implies high connectivity and synchronicity, which is crucial for obtaining good MEA analysis results.
Metric dependent response of Cardiosight®. The ratio of ventricular: atrial: nodular cells in Cardiosight® is 80:19:1, and the gap junction protein Connexin43 is accurately located between cells, with over 96% of cTnT positive expression.
Electrophysiology
The drug sensitivity of Cardiosight® is very high, making it easy to detect electrophysiological changes caused by drugs. E4031 (Ikr inhibitor) treatment showed prolonged FPD; Treatment with isoproterenol showed an increase in contraction amplitude.
The electrophysiology of Cardiosight® has been validated on multiple platforms, with highly consistent signal shapes and synchronous electrodes, making it suitable for high-throughput drug testing.
