Morphology
On the 30th day of Heart organoid® differentiation.
Features
cTnT
VE-Cad
DAPI
Vim
a-Act
DAPI
Heart organoid® expresses ventricular cardiomyocyte specific genes (cTnT, a-Act); endothelial cells (VE Cad); Fibroblasts (Vim).
Heart organoid® contains cardiomyocytes (51%); Fibroblasts (25%); Endothelial cells (14%).
Disease simulation
Heart organoid® simulates acute myocardial infarction (AMI) through ischemia reperfusion (IR), and then simulates heart failure by inducing fibrosis, reproducing the pathological characteristics of human patients.
AMI-Heart organoid® observed disruption of α-Actinin, which maintains the myocardial cytoskeleton, significantly reduced expression of cTnT and cTnl in organoids, and decreased electrophysiological function and Spike Amplitude.
Heart failure Heart organoid® observed collagen accumulation and prolongation of the electrophysiological activity potential of FPCcf, and arrhythmias were observed.
